CRISPR Injections

We inject the CRISPR constructs you provide to us.

our benefits.

Experience the advantages of our CRISPR Injection services, where precision meets efficiency in the realm of genome editing. Discover the benefits of our tailored solutions.

Visible Markers

We can screen for CRISPR positive animals via visible markers.

Scalable embryos

We can scale the number of injected embryos up or down if desired.

PCR Screening

We can screen via PCR. For this service, ask for a quote.

our services.

We offer various CRISPR injection services, such as gRNA, gRNA + donor, and gRNA + ssODN injections.

With this type of service, we will not review your CRISPR design nor evaluate your CRISPR constructs.

gRNA Injections

In its simplest form we inject gRNAs for creating knockouts (indels). We inject ~250 nosCas9 embryos.

Currently used CRISPR strains: nos-Cas9mSA (II, P40) and nos-Cas9mSA (III, P2).

gRNA + donor Injections

We further inject gRNA constructs together with a donor construct for generating various types of knockouts or knockins. Currently, we suggest injecting ~500 Cas9 embryos to likely get several independent insertions of the donor.

the pricing.

Our goal is to achieve success with the very first CRISPR injection, avoiding the need for re-injections resulting in time delays. For this reason we inject a relatively high number of embryos.

If the goal (knockout, knockin) is not achieved with the first injection, our use of high embryo numbers strongly indicates that the failure most likely originates in something entirely unrelated to the injection (such as design errors, gRNA issues, construct flaws…).

Injection Only
(gRNA)

230 CHF

Injection of ~250 nos-Cas9 embryos and shipment of the larvae.

Processing time:

~2 weeks

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Injection Only (gRNA + donor)

400 CHF

Injection of ~500 nos-Cas embryos with gRNAs + donor construct and shipment of the larvae.

Processing time:

~2 weeks

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F1 Transformants (gRNA + donor)

500 CHF

Injection of ~500 nos-Cas embryos with gRNAs + donor construct and screening for visible marker.

Processing time:

~6-7 weeks

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Balanced Stocks (gRNA + donor)

560 CHF

CRISPR positive flies will be balanced.

Processing time:

~9-10 weeks

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If you plan another type of CRISPR experiment or you want a variation of our offered services – please feel free to contact us.

FAQs

What strains do you use for the CRISPR/Cas9 injections?

Currently we mainly use two strains: nos-Cas9-mSA (P40, on 2^nd) and nos-Cas9-mSA (P2, on 3^rd chromosome). In these strains the Cas9 is fused to a monomeric streptavidin (cas9-mSA).

These Cas9-mSA strains are healthy and their offspring has a good survival rate.

Shall we send our samples for CRISPR/Cas9 injections as premix or as individual plasmids in case of a gRNA + donor injection?

We prefer sending individual plasmids so that we are flexible with the final concentrations. Additionally, we can react on potential DNA quality issues. However, both options are acceptable.

How much DNA should I send for CRISPR injections?

For CRISPR transgenesis please provide 30 μl or more of your gRNA construct(s) at a concentration of 500 ng/μl or higher. For donor constructs, we require an amount of 30 μl or more at a concentration of 1 μg/μl or higher.

If you want us to take care of the DNA you can just send us a small aliquot of miniprep DNA and we will prepare injection quality DNA in-house for CHF 30 per sample.

Do you provide a free re-injection if you don't get any transformants with this CRISPR service?

We provide no free re-injection for a failed CRISPR experiment when we are not in charge of the CRISPR design and the generation of the reagents. The reason for this is that in a CRISPR experiment there are many parameters that are relevant for its success such as the CRISPR design, selection and efficiency of gRNAs, cloning-related issues. When you send us the CRISPR reagents we cannot control for any of these parameters. In our experience the failure of a CRISPR experiment most likely depends on one of such parameters or a combination of them and generally not on the injection itself.