PhiC31
Insertion of your transgenic constructs into a collection of defined attP target sites.
our benefits.
PhiC31 services.
Injection Only
Balanced Stocks
Currently available for 2nd and 3rd chromosome. After injection the transformant flies will be crossed with an appropriate balancer strain (2nd: yw; If/CyO. 3rd: yw;; MKRS/TM6B). The balanced stocks will be shipped and we will maintain balanced stocks for at least 4 weeks.
F1 Transformants
We can screen for the following: white, yellow, vermilion, GFP, RFP or DsRed.
the pricing.
Prices listed are for constructs <20 kb.
For constructs >20 kb we will provide a quote for you.
Injection Only
–
210 CHF
- injection
- shipping of larvae
- transformants not identified
- integrase still present
- no backup strains
- no free re-injection
order now
F1 Transformants
(y+ & w+)
260 CHF
Outcross of the injected animals and screening for transformants.
- injection
- screening
- shipping of transformants
- integrase removed
- backup strains
order now
F1 Transformants
300 CHF
Outcross of the injected animals and screening for vermilion and fluorescent markers.
- injection
- screening
- shipping of transformants
- integrase removed
- backup strains
order now
Balanced Stocks
(y+ & w+)
300 CHF
- injection
- screening
- balancing
- shipping of balanced stocks
- integrase removed
- backup strains
order now
Balanced Stocks
350 CHF
- injection
- screening
- balancing
- shipping of balanced stocks
- integrase removed
- backup strains
order now
FAQs
How much DNA should I send for the PhiC31 Injection service?
For site-specific transgenesis please provide 30 μl or more of your DNA construct at a concentration of 300 ng/μl or higher. We will dilute the DNA to an appropriate injection concentration.
If you want us to take care of the DNA you can just send us a small aliquot of miniprep DNA and we will prepare injection quality DNA in-house for CHF 30.
What happens if you don’t get any transgenics with the phiC31 service?
We offer one free re-injection if you can confirm that the plasmid is ok and should not cause lethality in a transgenic situation.
Our experience is that close to 100% of our phiC31 injections work in the first attempt. In the rare cases where we did not get transgenics in the first round the reason was mostly a problem with the injected plasmid: the plasmid is not meant for phiC31 injection (it is missing an attB recombination site); the marker is damaged or of a different nature than originally specified; there is no plasmid present (e.g. due to degradation); the transgene is e.g. an oncogene and likely causes lethality when inserted, due to leaky expression at the insertion site (leaky expression at attP target sites is a common phenomenon).
In short, a re-injection of plasmids with such issues doesn’t help to get transgenics. Therefore, we recommend to first re-check and evaluate the plasmids before doing a re-injection. We can assist you with this.
